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Article

Effects of RNA polymerase modifications on transcription-induced negative supercoiling and associated R-loop formation.

Molecular microbiology - 1 Jun 2004

Broccoli Sonia, Rallu Fabien, Sanscartier Patrick, Cerritelli Susana M, Crouch Robert J, Drolet Marc

Abstract excerpt

Transcription in the absence of topoisomerase I, but in the presence of DNA gyrase, can result in the formation of hypernegatively supercoiled DNA and associated R-loops. In this paper, we have used several strategies to study the effects of elongation/termination properties of RNA polymerase on such transcription-induced supercoiling. Effects on R-loop formation were exacerbated when cells were exposed to...

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PMID: 15186424
DOI: 10.1111/j.1365-2958.2004.04092.x

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