Article
Neutralizing mutations of carboxylates that bind metal 2 in T5 flap endonuclease result in an enzyme that still requires two metal ions.
The Journal of biological chemistry - 2 Sept 2011
Tomlinson Christopher G, Syson Karl, Sengerová Blanka, Atack John M, Sayers Jon R, Swanson Linda, Tainer John A, Williams Nicholas H, Grasby Jane A
Abstract excerpt
Flap endonucleases (FENs) are divalent metal ion-dependent phosphodiesterases. Metallonucleases are often assigned a "two-metal ion mechanism" where both metals contact the scissile phosphate diester. The spacing of the two metal ions observed in T5FEN structures appears to preclude this mechanism. However, the overall reaction catalyzed by wild type (WT) T5FEN requires three Mg(2+) ions, implying that a third...
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