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Article

Discovery and directed evolution of a glyphosate tolerance gene.

Science (New York, N.Y.) - 21 May 2004

Castle Linda A, Siehl Daniel L, Gorton Rebecca, Patten Phillip A, Chen Yong Hong, Bertain Sean, Cho Hyeon-Je, Duck Nicholas, Wong James, Liu Donglong, Lassner Michael W

Abstract excerpt

The herbicide glyphosate is effectively detoxified by N-acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N-acetyltransferase (GAT) activity. Kinetic properties of the discovered enzymes were insufficient to confer glyphosate tolerance to transgenic organisms. Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four orders of magnitude...

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Identifiers

PMID: 15155947
DOI: 10.1126/science.1096770

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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