Article
Improving glyphosate oxidation activity of glycine oxidase from Bacillus cereus by directed evolution.
PloS one - 1 Jan 2013
Zhan Tao, Zhang Kai, Chen Yangyan, Lin Yongjun, Wu Gaobing, Zhang Lili, Yao Pei, Shao Zongze, Liu Ziduo
Abstract excerpt
Glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (GO) has been reported to be able to catalyze the oxidative deamination of various amines and cleave the C-N bond in glyphosate. Here, in an effort to improve the catalytic activity of the glycine oxidase that was cloned from a...
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