Article
A new type of class I bacterial 5-enopyruvylshikimate-3-phosphate synthase mutants with enhanced tolerance to glyphosate.
Biochimica et biophysica acta - 7 Nov 2001
He M, Yang Z Y, Nie Y F, Wang J, Xu P
Abstract excerpt
Glyphosate or Roundup is the most extensively used herbicide for broad-spectrum control of weeds. Glyphosate inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme in the aromatic amino acid biosynthetic pathway in microorganisms and plants. Applying the staggered extension process, we randomly mutated and recombined the aroA genes of Salmonella typhimurium and Escherichia coli to obtain four...
Topics
- 3-Phosphoshikimate 1-Carboxyvinyltransferase
- Alkyl and Aryl Transferases
- Amino Acid Sequence
- Dose-Response Relationship, Drug
- Drug Tolerance
- Enzyme Inhibitors
- Escherichia coli
- Glycine
- Kinetics
- Molecular Sequence Data
- Mutation
- Phosphoenolpyruvate
- Salmonella typhimurium
- Sequence Alignment
- Glyphosate