Article
Isolation of a CDC28 homologue from Cryptococcus neoformans that is able to complement cdc28 temperature-sensitive mutants of Saccharomyces cerevisiae.
FEMS yeast research - 1 May 2004
Takeo Kanji, Ogura Yasunobu, Virtudazo Eric, Raclavsky Vladislav, Kawamoto Susumu
Abstract excerpt
A partial cDNA fragment of the Cryptococcus neoformans homologue of the main cell cycle control gene CDC28/cdc2 was isolated using degenerate primer RT-PCR. A subsequent search in the C. neoformans genome database identified several sequences similar to CDC28/cdc2. A part of the sequence which showed the highest similarity to CDC28/cdc2 turned out to be identical to the partial cyclin-dependent kinase (Cdk) cDNA...
Topics
- Amino Acid Sequence
- Base Sequence
- CDC2 Protein Kinase
- CDC2-CDC28 Kinases
- CDC28 Protein Kinase, S cerevisiae
- Cryptococcus neoformans
- DNA, Fungal
- Genes, Fungal
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Phylogeny
- Saccharomyces cerevisiae