Article
Isolation and characterization of cDNA clones encoding cdc2 homologues from Oryza sativa: a functional homologue and cognate variants.
Molecular & general genetics : MGG - 1 May 1992
Hashimoto J, Hirabayashi T, Hayano Y, Hata S, Ohashi Y, Suzuka I, Utsugi T, Toh-e A, Kikuchi Y
Abstract excerpt
Using probes obtained by PCR amplification, we have isolated two cognate rice cDNAs (cdc2Os-1 and cdc2Os-2) encoding structural homologues of the cdc2+/CDC28 (cdc2) protein kinase from a cDNA library prepared from cultured rice cells. Comparison of the deduced amino acid sequences of cdc2Os-1 and cdc2Os-2 showed that they are 83% identical. They are 62% identical to CDC28 of Saccharomyces cerevisiae and much more...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- CDC2 Protein Kinase
- Cells, Cultured
- DNA
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation