Article
CDKA and CDKB kinases from Chlamydomonas reinhardtii are able to complement cdc28 temperature-sensitive mutants of Saccharomyces cerevisiae.
Protoplasma - 1 Jan 2008
Cízková M, Pichová A, Vítová M, Hlavová M, Hendrychová J, Umysová D, Gálová E, Sevcovicová A, Zachleder V, Umen J G, Bisová K
Abstract excerpt
Cyclin-dependent kinases (CDK) play a key role in coordinating cell division in all eukaryotes. We investigated the capability of cyclin-dependent kinases CDKA and CDKB from the green alga Chlamydomonas reinhardtii to complement a Saccharomyces cerevisiae cdc28 temperature-sensitive mutant. The full-length coding regions of algal CDKA and CDKB cDNA were amplified by RT-PCR and cloned into the yeast expression...
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