Article
Nanosecond dynamics of acetylcholinesterase near the active center gorge.
The Journal of biological chemistry - 18 Jun 2004
Boyd Aileen E, Dunlop Cristina S, Wong Lilly, Radic Zoran, Taylor Palmer, Johnson David A
Abstract excerpt
To delineate the role of peptide backbone flexibility and rapid molecular motion in acetylcholinesterase catalysis and inhibitor association, we investigated the decay of fluorescence anisotropy at three sites of fluorescein conjugation to cysteine-substitution mutants of the enzyme. One cysteine was placed in a loop at the peripheral site near the rim of the active center gorge (H287C); a second was in a helical...
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