Article
Stable and unstable transformation by microinjection of macronucleoplasm in Paramecium.
Developmental genetics - 1 Jan 1992
Harumoto T, Hiwatashi K
Abstract excerpt
Transformation by microinjection of macronucleoplasm in Paramecium caudatum was investigated. Macronucleoplasm with three genetic markers (behavior, trichocyst, and mating type) was injected into the macronucleus. To facilitate microinjection, in most cases, paramecia were immobilized in a gelatin (7.5%) solution. The injected cells began to express a dominant gene (cnrA+ or cnrB+) of the donor 9-24 hr after...
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