Article
Permanent rescue of a non-Mendelian mutation of Paramecium by microinjection of specific DNA sequences.
Genetics - 1 Nov 1991
Jessop-Murray H, Martin L D, Gilley D, Preer J R, Polisky B
Abstract excerpt
The mutant Paramecium tetraurelia cell line d48 is unable to express the serotype A protein on its surface. Although the A gene is intact in the micronuclei of d48, the A gene copies in the macronucleus contain a large deletion eliminating virtually the entire coding sequence. Previous studies showed that microinjection of a plasmid containing the entire A gene into the macronucleus of d48 permanently restored A...
Topics
- Animals
- Antigens, Surface
- DNA Mutational Analysis
- DNA, Protozoan
- Microinjections
- Mutation
- Paramecium
- Plasmids
- Protozoan Proteins
- RNA, Messenger
- RNA, Protozoan