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Cloning and sequencing of the ura3 and ura5 genes, and isolation and characterization of uracil auxotrophs of the fungus Mortierella alpina 1S-4.

Bioscience, biotechnology, and biochemistry - 1 Feb 2004

Takeno Seiki, Sakuradani Eiji, Murata Shoichi, Inohara-Ochiai Misa, Kawashima Hiroshi, Ashikari Toshihiko, Shimizu Sakayu

Abstract excerpt

The oil-producing fungus Mortierella alpina 1S-4 is an industrial strain. In order to prepare host strains for a transformation system for this fungus, six uracil auxotrophs were obtained by means of random mutation with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). When the activities of orotate phosphoribosyl transferase (OPRTase, EC 2.4.2.10) and orotidine-5'-phosphate decarboxylase (OMPdecase, EC 4.1.1.23)...

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PMID: 14981288
DOI: 10.1271/bbb.68.277

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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