Article
Establishment of an overall transformation system for an oil-producing filamentous fungus, Mortierella alpina 1S-4.
Applied microbiology and biotechnology - 1 Sept 2004
Takeno Seiki, Sakuradani Eiji, Murata Shoichi, Inohara-Ochiai Misa, Kawashima Hiroshi, Ashikari Toshihiko, Shimizu Sakayu
Abstract excerpt
Oil-producing fungus Mortierella alpina 1S-4 is an industrial strain. To determine its physiological properties and to clarify the biosynthetic pathways for polyunsaturated fatty acids, a transformation system for this fungus was established using a derivative of it, i.e., a ura5- mutant lacking orotate phosphoribosyl transferase (OPRTase, EC.2.4.2.10) activity. Transformation with a vector containing the...
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