Article
Phenotypic and functional analyses of CD34NEG hematopoietic precursors from mobilized peripheral blood.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2004
Dooley Douglas C, Oppenlander Barbara K
Abstract excerpt
Methods are described for the characterization of CD34 antigen modulation and its relationship to cell proliferation in early human hematopoietic cells. Toward that end, quiescent primitive CD34+ and CD34NEG cells are purified from mobilized peripheral blood (MoPB). Unlike CD34NEG cells from other sources, those from MoPB grow readily in stroma-free culture, facilitating their analysis. Using a lineage-depleted,...
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