Article
Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene.
PCR methods and applications - 1 Aug 1992
Cha R S, Zarbl H, Keohavong P, Thilly W G
Abstract excerpt
We have found that under appropriate conditions, an allele-specific polymerase chain reaction (PCR) can achieve a sensitivity suitable for measuring specific, infrequent mutations in single cell systems or in animal tissues. Using the 12th codon GC-to-AT mutation in the rat c-Ha-ras gene as a model system, we have defined conditions that allow for measurement of mutations present at frequencies as low as one in...
Topics
- Alleles
- Animals
- Base Sequence
- Codon
- DNA Mutational Analysis
- Genes, ras
- Molecular Sequence Data
- Polymerase Chain Reaction
- Rats
- Rats, Inbred F344
- Sensitivity and Specificity