Article
Chemical-genetic inhibition of a sensitized mutant myosin Vb demonstrates a role in peripheral-pericentriolar membrane traffic.
Proceedings of the National Academy of Sciences of the United States of America - 17 Feb 2004
Provance D William, Gourley Christopher R, Silan Colleen M, Cameron L C, Shokat Kevan M, Goldenring James R, Shah Kavita, Gillespie Peter G, Mercer John A
Abstract excerpt
Selective, in situ inhibition of individual unconventional myosins is a powerful approach to determine their specific physiological functions. Here, we report the engineering of a myosin Vb mutant that still hydrolyzes ATP, yet is selectively sensitized to an N(6)-substituted ADP analog that inhibits its activity, causing it to remain tightly bound to actin. Inhibition of the sensitized mutant causes inhibition...
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