Article
Chemical-genetic inhibition of sensitized mutant unconventional myosins.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007
Karcher Ryan L, Provance D William, Gillespie Peter G, Mercer John A
Abstract excerpt
The construction of a sensitized mutant unconventional myosin is an excellent method for determining the function of the individual myosin against a background of related myosins with partially overlapping functions. In this chapter, we outline the steps involved in sensitizing myosin by mutation and screening them against panels of nucleotide analogs, including transfection, microinjection, and actin...
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