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Article

Genetic modification of the penicillin G acylase surface to improve its reversible immobilization on ionic exchangers.

Applied and environmental microbiology - 1 Jan 2007

Montes Tamara, Grazú Valeria, López-Gallego Fernando, Hermoso Juan A, García Jose L, Manso Isabel, Galán Beatriz, González Ramón, Fernández-Lafuente Roberto, Guisán José M

Abstract excerpt

A new mutant of the industrial enzyme penicillin G acylase (PGA) from Escherichia coli has been designed to improve its reversible immobilization on anionic exchangers (DEAE- or polyethyleneimine [PEI]-coated agarose) by assembling eight new glutamic residues distributed homogeneously through the enzyme surface via site-directed mutagenesis. The mutant PGA is produced and processed in vivo as is the native...

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Identifiers

PMID: 17098917
DOI: 10.1128/AEM.02107-06
PMCID: PMC1797127

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Source receipt: pubmed, retrieved 7 Sept 2026.

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