Article
Detection of isolated tumor cells by polymerase chain reaction-restriction fragment length polymorphism for K-ras mutations in tissue samples of 199 colorectal cancer patients.
Clinical cancer research : an official journal of the American Association for Cancer Research - 15 Jan 2004
Dieterle Christoph P, Conzelmann Michael, Linnemann Ulrich, Berger Martin R
Abstract excerpt
The aim of this study was to identify K-ras mutations as marker for isolated tumor cells in liver, lymph node, and bone marrow specimens of colorectal cancer patients. To detect these, a PCR-RFLP assay was used with a sensitivity exceeding that of routine histopathology by at least 1 order of magnitude. In addition, the ratio of mutated versus wild-type alleles was determined by an internal standard. Of 199...
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