Article
Role of the NH2 terminus in the assembly and trafficking of the intermediate conductance Ca2+-activated K+ channel hIK1.
The Journal of biological chemistry - 9 Apr 2004
Jones Heather M, Hamilton Kirk L, Papworth Glenn D, Syme Colin A, Watkins Simon C, Bradbury Neil A, Devor Daniel C
Abstract excerpt
The role of the NH(2)-terminal leucine zipper and dileucine motifs of hIK1 in the assembly, trafficking, and function of the channel was investigated using cell surface immunoprecipitation, co-immunoprecipitation (Co-IP), immunoblot, and whole-cell patch clamp techniques. Mutation of the NH(2)-terminal leucine zipper at amino acid positions 18 and 25 (L18A/L25A) resulted in a complete loss of steady-state protein...
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