Article
[Establishment of a method with micro-channel electrophoresis for detecting the mutations of isoniazid-resistant genes in Mycobacterium tuberculosis].
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases - 1 Dec 2003
Wang Ji, Cui Zhen-ling, Jing Feng-xiang, Jin Qing-hui, Zhao Jian-long, Hu Zhong-yi, Xu Yuan-sen
Abstract excerpt
OBJECTIVE: To establish a method for the detection of isoniazid resistance-associated mutations in katG gene and inhA gene in Mycobacterium tuberculosis with single-stranded conformation polymorphism (SSCP) analysis by the micro-channel electrophoresis chip system. METHODS: The polymer solutions of acrylamide and its derivatives were used for sieving matrices with small amount of acridine orange as the...
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