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Article

Efficient switching of mycobacteriophage L5-based integrating plasmids in Mycobacterium tuberculosis.

FEMS microbiology letters - 12 Dec 2003

Pashley Carey A, Parish Tanya

Abstract excerpt

We previously used a mycobacteriophage L5-derived integrating vector to demonstrate that glnE and aroK are essential genes in Mycobacterium tuberculosis by showing that we were unable to excise the integrated vector when it carried the only functional copy of these genes. We tested three systems to replace the integrated copy with alternative alleles. The most efficient method was to transform the strain with a...

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Identifiers

PMID: 14680701
DOI: 10.1016/S0378-1097(03)00823-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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