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Article

Identification of crucial histidines for heme binding in the N-terminal domain of the heme-regulated eIF2alpha kinase.

The Journal of biological chemistry - 20 Feb 2004

Inuzuka Takayuki, Yun Bo-Geon, Ishikawa Haruto, Takahashi Satoshi, Hori Hiroshi, Matts Robert L, Ishimori Koichiro, Morishima Isao

Abstract excerpt

The heme-regulated eukaryotic initiation factor-2alpha (eIF2alpha) kinase (HRI) regulates the initiation of protein synthesis in reticulocytes. The binding of NO to the N-terminal heme-binding domain (NTD) of HRI positively modulates its kinase activity. By utilizing UV-visible absorption, resonance Raman, EPR and CD spectroscopies, two histidine residues have been identified that are crucial for the binding of...

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PMID: 14672943
DOI: 10.1074/jbc.C300464200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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