Article
On/off switch mediated by Exo+ polymerases: experimental analysis for its physiological and technological implications.
Journal of biochemistry and molecular biology - 30 Nov 2003
Zhang Jia, Chen Lin-Ling, Guo Zi-Fen, Peng Cui-Ying, Liao Duan-Fang, Li Kai
Abstract excerpt
The potential physiological role and technological application of the premature termination of DNA polymerization through the off-switch of exo+ polymerases were studied using 3' phosphorothioate-modified or unmodified primers with single base mismatch distal to the 3' terminus. With exonuclease-digestible unmodified primers, a gradient premature termination of DNA polymerization was observed when amplified with...
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