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Effect of Timing of the Removal of Oocyte Chromosomes Before or After Injection of Somatic Nucleus on Development of NT Embryos

1 Sept 2003

Abstract excerpt

Cloning methods are now well described and becoming routine. Yet the frequency at which live cloned offspring are produced (as a percentage of starting one-cell embryos) remains below 5% irrespective of nucleus donor species or cell type. In considering the cause(s) of this universally low efficiency, features common to all cloning protocols are strong candidates. One such shared feature is enucleation; the donor...

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PMID: 14588136
DOI: 10.1089/153623003769645848

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