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Article

Direct Exposure of Chromosomes to Nonactivated Ovum Cytoplasm Is Effective for Bovine Somatic Cell Nucleus Reprogramming1

1 Jan 2001

Abstract excerpt

We examined the in vitro developmental potential of nonactivated and activated enucleated ova receiving cumulus cells at various stages of the cell cycle. Eleven to 29% of activated ova receiving donor cells stopped developing at the 8-cell stage but 21% to 50% of nonactivated ova receiving donor cells at either the G(0), G(1), G(2), or M phase, or cycling cells developed into blastocysts. One normal calf was...

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PMID: 11133690
DOI: 10.1095/biolreprod64.1.324

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