Article
Nucleotide binding by the Mdm2 RING domain facilitates Arf-independent Mdm2 nucleolar localization.
Molecular cell - 1 Oct 2003
Poyurovsky Masha V, Jacq Xavier, Ma Charles, Karni-Schmidt Orit, Parker Peter J, Chalfie Martin, Manley James L, Prives Carol
Abstract excerpt
The RING domain of Mdm2 contains a conserved Walker A or P loop motif that is a characteristic of nucleotide binding proteins. We found that Mdm2 binds adenine-containing nucleotides preferentially and that nucleotide binding leads to a conformational change in the Mdm2 C terminus. Although nucleotide binding is not required for Mdm2 E3 ubiquitin ligase activity, we show that nucleotide binding-defective P loop...
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