Article
Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolytic activity of CvaB, an ATP-binding cassette transporter.
The Journal of biological chemistry - 9 Jan 2004
Wu Kai-Hui, Tai Phang C
Abstract excerpt
CvaB, a member of the ATP-binding cassette transporter superfamily, is the central membrane transporter of the colicin V secretion system in Escherichia coli. Cys32 and His105 in the N-terminal domain of CvaB were identified as critical residues for both colicin V secretion and cysteine proteolytic activity. By inhibiting degradation with N-ethylmaleimide and a mixture of protease inhibitors, a stable wild-type...
Topics
- ATP-Binding Cassette Transporters
- Amino Acid Sequence
- Animals
- Blotting, Western
- Calcium
- Calpain
- Cell Division
- Colicins
- Culture Media
- Cysteine
- Cysteine Endopeptidases