Article
Purification and characterization of colicin V from Escherichia coli culture supernatants.
Biochemistry - 7 Jun 1994
Fath M J, Zhang L H, Rush J, Kolter R
Abstract excerpt
The peptide antibiotic, colicin V (ColV), has been purified and characterized from Escherichia coli culture supernatants by precipitation with trichloroacetic acid (TCA) and high-performance liquid chromatography (HPLC). Polyacrylamide gel electrophoresis (PAGE) and Western analysis identifies ColV as a polypeptide with an apparent molecular mass of 5.8 kDa. The protein identified remains biologically active...
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