Purification and characterization of colicin V from Escherichia coli culture supernatants.

doi:10.1021/bi00188a021

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Article

Purification and characterization of colicin V from Escherichia coli culture supernatants.

Biochemistry - 7 Jun 1994

Fath M J, Zhang L H, Rush J, Kolter R

Abstract excerpt

The peptide antibiotic, colicin V (ColV), has been purified and characterized from Escherichia coli culture supernatants by precipitation with trichloroacetic acid (TCA) and high-performance liquid chromatography (HPLC). Polyacrylamide gel electrophoresis (PAGE) and Western analysis identifies Co...

Topics

Amino Acid Sequence
Blotting, Western
Chemical Precipitation
Chromatography, High Pressure Liquid
Colicins
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Molecular Sequence Data
Mutation
Sequence Homology, Amino Acid
Trichloroacetic Acid

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