Article
Structural analysis of wild-type and mutant yeast calmodulins by limited proteolysis and electrospray ionization mass spectrometry.
Protein science : a publication of the Protein Society - 1 Apr 1992
Brockerhoff S E, Edmonds C G, Davis T N
Abstract excerpt
Calmodulin from Saccharomyces cerevisiae was expressed in Escherichia coli and purified. The purified protein was structurally characterized using limited proteolysis followed by ESI mass spectrometry to identify the fragments. In the presence of Ca2+, yeast calmodulin is sequentially cleaved at...
Topics
- Calcium
- Calmodulin
- Escherichia coli
- Mass Spectrometry
- Mutation
- Peptide Fragments
- Recombinant Proteins
- Saccharomyces cerevisiae