Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

High-level expression of porcine liver cytochrome P-450 reductase catalytic domain in Escherichia coli by modulating the predicted local secondary structure of mRNA.

Journal of biochemistry - 1 Sept 2003

Kimura Shigenobu, Iyanagi Takashi

Abstract excerpt

A direct expression system for the solubilized catalytic domains of NADPH-cytochrome P-450 reductase (sCPR) from rat (RsCPR) and porcine (PsCPR) in Escherichia coli cells was constructed using the expression plasmid pCWori(+). PsCPR was minimally expressed, whereas RsCPR was highly expressed. Replacement of the nucleotides encoding Thr(60)Ser(61)Ser(62) in PsCPR with those for Ala(60)Pro(61)Pro(62) in RsCPR...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Escherichia coli
Share to Liver
Share to NADPH-Ferrihemoprotein Reductase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Plasmids

Share to Recombinant Fusion Proteins

Share to Structure-Activity Relationship

Source

Identifiers

PMID: 14561726
DOI: 10.1093/jb/mvg158

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 9 closest

High-level expression of porcine liver cytochrome P-450 reductase catalytic domain in Escherichia coli by modulating the predicted local secondary structure of mRNA.PMID 14561726Read ↗

Systematic mutations of highly conserved His49 and carboxyl-terminal of recombinant porcine liver NADH-cytochrome b5 reductase solubilized domain.Semantic proximity Read ↗

Optimizations to achieve high-level expression of cytochrome P450 proteins using Escherichia coli expression systems.Semantic proximity Read ↗

High-level expression of pig liver thioltransferase (glutaredoxin) in Escherichia coli.Semantic proximity Read ↗

Engineering the CYP101 system for in vivo oxidation of unnatural substrates.Semantic proximity Read ↗

Escherichia coli peptide methionine sulfoxide reductase gene: regulation of expression and role in protecting against oxidative damage.Semantic proximity Read ↗

Effects of flavin-binding motif amino acid mutations in the NADH-cytochrome b5 reductase catalytic domain on protein stability and catalysis.Semantic proximity Read ↗

Molecular basis for cell-specific regulation of the NADPH-cytochrome P450 oxidoreductase gene.Semantic proximity Read ↗

Putidaredoxin reductase and putidaredoxin. Cloning, sequence determination, and heterologous expression of the proteins.Semantic proximity Read ↗

Putative functions of phenylalanine-350 of Pseudomonas putida cytochrome P-450cam.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.