Putidaredoxin reductase and putidaredoxin. Cloning, sequence determination, and heterologous expression of the proteins.

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Article

Putidaredoxin reductase and putidaredoxin. Cloning, sequence determination, and heterologous expression of the proteins.

The Journal of biological chemistry - 15 Apr 1990

Peterson J A, Lorence M C, Amarneh B

Abstract excerpt

The oxidation of camphor by cytochrome P-450cam requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P-450 for oxygen activation. A 2.2-kilobase pair BamHI-StuI fragment from whole cell DNA of camphor-grown Pseudomonas putida has been cloned and sequenced. Translation of the sequence revealed two open...

Topics

Amino Acid Sequence
Base Sequence
Blotting, Southern
Cloning, Molecular
Codon
DNA, Bacterial
Escherichia coli
Ferredoxins
Gene Library
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

NADH, NADPH Oxidoreductases

Nucleic Acid Hybridization

Oligonucleotide Probes

Plasmids

Pseudomonas

Recombinant Proteins

Restriction Mapping

Source

Open in PubMed

Identifiers

PMID: 2180940

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2180940-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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