Article
Engineering the CYP101 system for in vivo oxidation of unnatural substrates.
Protein engineering - 1 Oct 2001
Bell S G, Harford-Cross C F, Wong L L
Abstract excerpt
The protein engineering of CYP enzymes for structure-activity studies and the oxidation of unnatural substrates for biotechnological applications will be greatly facilitated by the availability of functional, whole-cell systems for substrate oxidation. We report the construction of a tricistronic plasmid that expresses the CYP101 monooxygenase from Pseudomonas putida, and its physiological electron transfer...
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