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Article

High-level expression of pig liver thioltransferase (glutaredoxin) in Escherichia coli.

The Journal of biological chemistry - 5 Jan 1990

Yang Y F, Wells W W

Abstract excerpt

We report the first high-level expression of a mammalian thioltransferase (glutaredoxin) in Escherichia coli. A NcoI site (CCATGG) was introduced into the cDNA encoding pig liver thioltransferase (glutaredoxin) by site-directed mutagenesis, in which the first G of the original sequence, GCATGG, was replaced by a C. The altered cDNA was cloned into an expression vector, plasmid pKK233-2, between the unique NcoI...

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PMID: 2403567

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