High-level expression of pig liver thioltransferase (glutaredoxin) in Escherichia coli.

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Article

High-level expression of pig liver thioltransferase (glutaredoxin) in Escherichia coli.

The Journal of biological chemistry - 5 Jan 1990

Yang Y F, Wells W W

Abstract excerpt

We report the first high-level expression of a mammalian thioltransferase (glutaredoxin) in Escherichia coli. A NcoI site (CCATGG) was introduced into the cDNA encoding pig liver thioltransferase (glutaredoxin) by site-directed mutagenesis, in which the first G of the original sequence, GCATGG, was replaced by a C. The altered cDNA was cloned into an expression vector, plasmid pKK233-2, between the unique NcoI...

Topics

Amino Acids
Animals
Base Sequence
Cloning, Molecular
DNA
Escherichia coli
Gene Expression
Glutaredoxins
Hydrogen-Ion Concentration
Immunoblotting
Isoelectric Focusing
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Liver

Mutation

Oxidoreductases

Plasmids

Proteins

Recombinant Proteins

Source

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Identifiers

PMID: 2403567

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2403567-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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