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Article

Putative functions of phenylalanine-350 of Pseudomonas putida cytochrome P-450cam.

Biochimica et biophysica acta - 11 Jan 1994

Yasukochi T, Okada O, Hara T, Sagara Y, Sekimizu K, Horiuchi T

Abstract excerpt

Cytochrome P-450cam hydroxylates d-camphor, using molecular oxygen and reducing equivalents transferred via putidaredoxin. We constructed mutant genes in which Phe-350 of P-450cam was replaced by Leu, Tyr, or His by site-directed mutagenesis, expressed them in Escherichia coli, purified the mutant proteins, and compared their enzymic properties with those of the wild type P-450cam. NADH oxidation rate of the Tyr...

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Identifiers

PMID: 8305479
DOI: 10.1016/0167-4838(94)90036-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Putative functions of phenylalanine-350 of Pseudomonas putida cytochrome P-450cam.PMID 8305479Read ↗

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