Article
Putative functions of phenylalanine-350 of Pseudomonas putida cytochrome P-450cam.
Biochimica et biophysica acta - 11 Jan 1994
Yasukochi T, Okada O, Hara T, Sagara Y, Sekimizu K, Horiuchi T
Abstract excerpt
Cytochrome P-450cam hydroxylates d-camphor, using molecular oxygen and reducing equivalents transferred via putidaredoxin. We constructed mutant genes in which Phe-350 of P-450cam was replaced by Leu, Tyr, or His by site-directed mutagenesis, expressed them in Escherichia coli, purified the mutant proteins, and compared their enzymic properties with those of the wild type P-450cam. NADH oxidation rate of the Tyr...
Topics
- Base Sequence
- Cytochrome P-450 Enzyme System
- Electron Transport
- Escherichia coli
- Ferredoxins
- Heme
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Phenylalanine
- Pseudomonas putida