Article
Functional cloning of the miltefosine transporter. A novel P-type phospholipid translocase from Leishmania involved in drug resistance.
The Journal of biological chemistry - 12 Dec 2003
Pérez-Victoria F Javier, Gamarro Francisco, Ouellette Marc, Castanys Santiago
Abstract excerpt
The antitumor drug miltefosine (hexadecylphosphocholine, MIL) has recently been approved as the first oral agent for the treatment of visceral leishmaniasis. Little is known about the mechanisms of action and uptake of MIL in either parasites or tumor cell lines. We have cloned a putative MIL transporter (LdMT) by functional rescue, using a Leishmania donovani-resistant line defective in the inward-directed...
Topics
- Adenosine Triphosphatases
- Alleles
- Amino Acid Sequence
- Animals
- Antiprotozoal Agents
- Biological Transport
- Blotting, Southern
- Carrier Proteins
- Cell Membrane
- Chromosome Mapping
- Cloning, Molecular
- Cosmids
- Dose-Response Relationship, Drug