Article
Mutations in the RNase H domain of HIV-1 reverse transcriptase affect the initiation of DNA synthesis and the specificity of RNase H cleavage in vivo.
Proceedings of the National Academy of Sciences of the United States of America - 9 Jul 2002
Julias John G, McWilliams Mary Jane, Sarafianos Stefan G, Arnold Edward, Hughes Stephen H
Abstract excerpt
Retroviral reverse transcriptases contain a DNA polymerase activity that can copy an RNA or DNA template and an RNase H activity that degrades the viral RNA genome during reverse transcription. RNase H makes both specific and nonspecific cleavages; specific cleavages are used to generate and remove the polypurine tract primer used for plus-strand DNA synthesis and to remove the tRNA primer used for minus-strand...
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