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Article

Directed evolution of xylanase J from alkaliphilic Bacillus sp. strain 41M-1: restore of alkaliphily of a mutant with an acidic pH optimum.

Nucleic acids research. Supplement (2001) - 1 Jan 2003

Inami Mayuko, Morokuma Chihiro, Sugio Akiko, Tamanoi Hidenori, Yatsunami Rie, Nakamura Satoshi

Abstract excerpt

Alkaliphilic Bacillus sp. strain 41M-1 produces an alkaliphilic xylanase (xylanase J). The newly constructed mutant E177Q deltaJC had an acidic pH optimum and showed almost no activity at pH 8.0. The alkaliphily of the enzyme was restored by directed evolution. The evolved mutants, Y176S/E177Q deltaJC and G32V/Y176D/E177Q deltaJC, retained about 30% and 43% activity of their maximal activities at pH 6.0,...

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PMID: 14510507
DOI: 10.1093/nass/3.1.315

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