Article
A calcium-dependent xylan-binding domain of alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1.
Bioscience, biotechnology, and biochemistry - 1 Jan 2011
Yazawa Risa, Takakura Jun, Sakata Tomoko, Ihsanawati, Yatsunami Rie, Fukui Toshiaki, Kumasaka Takashi, Tanaka Nobuo, Nakamura Satoshi
Abstract excerpt
Xylanase J of alkaliphilic Bacillus sp. strain 41M-1 contains a carbohydrate-binding module family 36 xylan-binding domain (XBD). Mutational analysis of the XBD revealed that Tyr237, Asp313, Trp317, and Asp318 were involved in Ca(2+)-dependent xylan-binding, and that Asp313 and Asp318 were especially important.
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