Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET).

Biochemical Society transactions - 1 Oct 2003

Lidke D S, Nagy P, Barisas B G, Heintzmann R, Post J N, Lidke K A, Clayton A H A, Arndt-Jovin D J, Jovin T M

Abstract excerpt

We report the implementation and exploitation of fluorescence polarization measurements, in the form of anisotropy fluorescence lifetime imaging microscopy (rFLIM) and energy migration Förster resonance energy transfer (emFRET) modalities, for wide-field, confocal laser-scanning microscopy and flow cytometry of cells. These methods permit the assessment of rotational motion, association and proximity of cellular...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Anisotropy
Share to CHO Cells

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cricetinae

Share to Green Fluorescent Proteins

Share to Luminescent Proteins

Share to Microscopy, Confocal

Share to Microscopy, Fluorescence

Share to Models, Statistical

Source

Identifiers

PMID: 14505472
DOI: 10.1042/bst0311020

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET).PMID 14505472Read ↗

Confocal fluorescence anisotropy and FRAP imaging of α-synuclein amyloid aggregates in living cells.Semantic proximity Read ↗

Intermolecular Energy Migration via HomoFRET Captures the Modulation in the Material Property of Phase-Separated Biomolecular CondensatesSemantic proximity Read ↗

Flow cytometric analysis of fluorescence resonance energy transfer: a tool for high-throughput screening of molecular interactions in living cells.Semantic proximity Read ↗

An improved Ras sensor for highly sensitive and quantitative FRET-FLIM imaging.Semantic proximity Read ↗

Multiplexed biochemical imaging reveals the extent and complexity of non-genetic heterogeneity in DNA damage-induced caspase dynamicsSemantic proximity Read ↗

A fluorescence polarization assay for identifying ligands that bind to vascular endothelial growth factor.Semantic proximity Read ↗

FRET and LRET Biosensors for Cell-based Imaging and Screening of Rac1 ActivationSemantic proximity Read ↗

A RasGTP-induced conformational change in C-RAF is essential for accurate molecular recognition.Semantic proximity Read ↗

Imaging FRET between spectrally similar GFP molecules in single cells.Semantic proximity Read ↗

Fluorescence correlation spectroscopy and its potential for intracellular applications.Semantic proximity Read ↗

Studies of receptor tyrosine kinase transmembrane domain interactions: the EmEx-FRET method.Semantic proximity Read ↗

Energy transfer efficiency on lymphocytes of leukemia patients measured by fluorescence polarization.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.