Article
Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication.
The Journal of cell biology - 29 Sept 2003
Kilmartin John V
Abstract excerpt
Centrins are calmodulin-like proteins present in microtubule-organizing centers. The Saccharomyces cerevisiae centrin, Cdc31p, was functionally tagged with a single Z domain of protein A, and used in pull-down experiments to isolate Cdc31p-binding proteins. One of these, Sfi1p, localizes to the half-bridge of the spindle pole body (SPB), where Cdc31p is also localized. Temperature-sensitive mutants in SFI1 show a...
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