Article
Reversal of the malignant phenotype by an anti-ras ribozyme.
Antisense research and development - 1 Jan 1992
Kashani-Sabet M, Funato T, Tone T, Jiao L, Wang W, Yoshida E, Kashfinn B I, Shitara T, Wu A M, Moreno J G
Abstract excerpt
In this study a ribozyme (catalytic RNA) was designed to site specifically cleave the mRNA of the activated H-ras gene expressed in human bladder carcinoma EJ cells. The optimal conditions for catalytic cleavage by the ribozyme were demonstrated in vitro. A synthetic DNA encoding the ribozyme was...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Cell Line
- Cloning, Molecular
- Genes, Synthetic
- Genes, ras
- Genetic Vectors
- Humans
- Mice
- Mice, Nude
- Molecular Sequence Data
- Neoplasm Transplantation
- Nucleic Acid Conformation
- Oligodeoxyribonucleotides
- Oligonucleotides, Antisense
- Phenotype