Article
Isolation and characterization of the Schizosaccharomyces pombe rad3 gene, involved in the DNA damage and DNA synthesis checkpoints.
Gene - 21 Sept 1992
Seaton B L, Yucel J, Sunnerhagen P, Subramani S
Abstract excerpt
We have cloned the Schizosaccharomyces pombe rad3 gene which is involved in G2 arrest following DNA damage, and in the dependence of mitosis on the completion of DNA replication. The gene was cloned by complementation of the sensitivity to UV light and gamma rays of the rad3-136 mutant with an Sz. pombe genomic library. Sublocalization of the complementing activity and sequencing of the clone identified an...
Topics
- Base Sequence
- Cloning, Molecular
- DNA Damage
- DNA, Fungal
- G2 Phase
- Genes, Fungal
- Genetic Complementation Test
- Genomic Library
- Molecular Sequence Data
- Phenotype
- Plasmids