Article
Mutations of p53 gene and their relation to disease progression in B-cell lymphoma.
Blood - 15 May 1992
Ichikawa A, Hotta T, Takagi N, Tsushita K, Kinoshita T, Nagai H, Murakami Y, Hayashi K, Saito H
Abstract excerpt
The alteration of p53 tumor suppressor gene was studied in 48 patients with B-cell lymphoma. A sequential combined technique of polymerase chain reaction-mediated single-strand conformational polymorphism (PCR-SSCP) or reverse transcription (RT)-PCR-SSCP and direct sequencing were used as a simple and sensitive approach to analyze nucleotide changes. By these methods, we identified 8 missense point mutations and...
Topics
- Base Sequence
- DNA, Neoplasm
- Exons
- Female
- Genes, p53
- Humans
- Lymph Nodes
- Lymphoma, B-Cell
- Male
- Middle Aged
- Molecular Sequence Data
- Mutation