Application of the standard mutagenesis assay results in underestimation of ethyl methanesulphonate-induced mutations to ouabain-resistance in Chinese hamster cells.

Abstract excerpt

Chinese hamster V79 cells were exposed to ethyl methanesulphonate (EMS) and the incidence of mutant cells resistant to 8-azaguanine (8AZG), 6-thioguanine (6TG) or ouabain (OUA) was determined both by the respreading and the in situ techniques. In the former assay, the mutagen-treated cultures were grown for several days to permit the expression of mutations after which the cells were trypsinized, replated (10(5)...

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