Article
Application of the standard mutagenesis assay results in underestimation of ethyl methanesulphonate-induced mutations to ouabain-resistance in Chinese hamster cells.
Mutation research - 1 May 1992
Mirzayans F, Parry J M, Mirzayans R
Abstract excerpt
Chinese hamster V79 cells were exposed to ethyl methanesulphonate (EMS) and the incidence of mutant cells resistant to 8-azaguanine (8AZG), 6-thioguanine (6TG) or ouabain (OUA) was determined both by the respreading and the in situ techniques. In the former assay, the mutagen-treated cultures were grown for several days to permit the expression of mutations after which the cells were trypsinized, replated (10(5)...
Topics
- Animals
- Azaguanine
- Cells, Cultured
- Cricetinae
- Drug Resistance
- Ethyl Methanesulfonate
- Mutagenicity Tests
- Mutation
- Ouabain
- Thioguanine