Article
Transfer of genes for utilization of starch (sta2) and melibiose (mel) to industrial strains of Saccharomyces cerevisiae by single-chromosome transfer, using a kar1 mutant as vector.
Applied microbiology and biotechnology - 1 May 1992
Spencer J F, Spencer D M, de Figueroa L, Nougues J M, Heluane H
Abstract excerpt
A method has been developed for the transfer of genes from other yeast strains and species to industrial yeast strains, using a haploid, kar1-1 mutant strain of Saccharomyces cerevisiae as a vector. The sta2 gene, conferring the ability to metabolize starch was transferred from an auxotrophic haploid strain of S. cerevisiae (S. diastaticus) and the melibiose-metabolism (mel) gene(s), from S. kluyveri, to the...
Topics
- Chromosomes, Fungal
- Electrophoresis
- Fungal Proteins
- Galactosidases
- Genes, Fungal
- Genetic Vectors
- Glucan 1,4-alpha-Glucosidase
- Melibiose
- Mutation
- Nuclear Proteins