Article
Transgenic production of a variant of human tissue-type plasminogen activator in goat milk: generation of transgenic goats and analysis of expression.
Bio/technology (Nature Publishing Company) - 1 Sept 1991
Ebert K M, Selgrath J P, DiTullio P, Denman J, Smith T E, Memon M A, Schindler J E, Monastersky G M, Vitale J A, Gordon K
Abstract excerpt
We report the first successful production of transgenic goats that express a heterologous protein in their milk. The production of a glycosylation variant of human tPA (LAtPA--longer acting tissue plasminogen activator) from an expression vector containing the murine whey acid promoter (WAP) operatively linked to the cDNA of a modified version of human tPA was examined in transgenic dairy goats. Two transgenic...
Topics
- Animals
- Animals, Genetically Modified
- Blotting, Southern
- DNA
- Embryo Transfer
- Female
- Gene Expression
- Genetic Variation
- Goats
- Humans
- Milk