Article
The D-galactose dehydrogenase gene from Pseudomonas fluorescens: characterization of mutations leading to increased expression in Escherichia coli.
Applied microbiology and biotechnology - 1 Jul 1990
Fiedler S, Buckel P
Abstract excerpt
The gene encoding D-galactose dehydrogenase (gld; E.C. 1.1.1.48) from Pseudomonas fluorescens is poorly expressed when cloned into Escherichia coli. Mutagenesis of the wild-type construct leads to a strong expression of gld in the heterologous host. To investigate the mutational events directing the increase in expression we constructed a gld-lacZ translational fusion which facilitated the isolation of mutants by...
Topics
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Galactose Dehydrogenases
- Gene Expression Regulation, Enzymologic
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Plasmids
- Pseudomonas fluorescens