Article
Chromosomal insertion of the entire Escherichia coli lactose operon, into two strains of Pseudomonas, using a modified mini-Tn5 delivery system.
Gene - 28 Feb 1997
Hansen L H, Sørensen S J, Jensen L B
Abstract excerpt
A 12-kb PstI fragment including the entire E. coli lactose operon (lacIPOZYA) was inserted in one copy into the chromosome of Pseudomonas putida, Pseudomonas fluorescens and an E. coli strain with lac- phenotype. This was made possible by improvements of an already existing mini-Tn5 transposon de...
Topics
- Chromosomes, Bacterial
- DNA Transposable Elements
- Escherichia coli
- Genotype
- Isopropyl Thiogalactoside
- Kinetics
- Lac Operon
- Mutagenesis, Insertional
- Phenotype
- Plasmids
- Pseudomonas fluorescens
- Pseudomonas putida
- Restriction Mapping