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Article

Cloning and nucleotide sequence of the glpD gene encoding sn-glycerol-3-phosphate dehydrogenase of Pseudomonas aeruginosa.

Journal of bacteriology - 1 Apr 1994

Schweizer H P, Po C

Abstract excerpt

Nitrosoguanidine-induced Pseudomonas aeruginosa mutants which were unable to utilize glycerol as a carbon source were isolated. By utilizing PAO104, a mutant defective in glycerol transport and sn-glycerol-3-phosphate dehydrogenase (glpD), the glpD gene was cloned by a phage mini-D3112-based in vivo cloning method. The cloned gene was able to complement an Escherichia coli glpD mutant. Restriction analysis and...

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Identifiers

PMID: 8157588
DOI: 10.1128/jb.176.8.2184-2193.1994
PMCID: PMC205338

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Source receipt: pubmed, retrieved 7 Sept 2026.

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